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lympholyte mamma density gradient medium  (Cedarlane)


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    Cedarlane lympholyte mamma density gradient medium
    Lympholyte Mamma Density Gradient Medium, supplied by Cedarlane, used in various techniques. Bioz Stars score: 95/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lympholyte+%C2%AE+-h+density+gradient+separation+medium/pmc12111189-73-8-14?v=Cedarlane
    Average 95 stars, based on 416 article reviews
    lympholyte mamma density gradient medium - by Bioz Stars, 2026-07
    95/100 stars

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    lympholyte density gradient separation medium  (Cedarlane)
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    2-D gels of caveolar-raft fractions. Silver-stained 2-D gels of caveolar-raft membrane fractions from control (A) and VEGF-stimulated ECFCs (VEGF: 50 ng/ml for 12 h) (B). Proteins (40 μg) were <t>separated</t> by their pI on 4–7 linear IPG strips and by their molecular weight on 9–16% polyacrylamide linear <t>gradient</t> gels. Black circles indicate spots that presented a differential occurrence in micro-domain after VEGF stimulation. Numbers indicate spot identified by mass spectrometry. The insets of each panel show the Western blotting with anticaveolin, antiflotillin and anti-integrinβ1 antibodies of fractions obtained by <t>density-gradient</t> centrifugation of ECFCs lysates (the caveolin-rich fractions Western blotting in each panel is representative of 20 different experiments performed with preparative purposes, to collect the amount of material to be subjected to mass-spectrometry analysis). Caveolin and flotillin positive fractions were collected and used in the 2-D gels. Molecular weights are reported on the right.
    Lympholyte Density Gradient Separation Medium, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2-D gels of caveolar-raft fractions. Silver-stained 2-D gels of caveolar-raft membrane fractions from control (A) and VEGF-stimulated ECFCs (VEGF: 50 ng/ml for 12 h) (B). Proteins (40 μg) were separated by their pI on 4–7 linear IPG strips and by their molecular weight on 9–16% polyacrylamide linear gradient gels. Black circles indicate spots that presented a differential occurrence in micro-domain after VEGF stimulation. Numbers indicate spot identified by mass spectrometry. The insets of each panel show the Western blotting with anticaveolin, antiflotillin and anti-integrinβ1 antibodies of fractions obtained by density-gradient centrifugation of ECFCs lysates (the caveolin-rich fractions Western blotting in each panel is representative of 20 different experiments performed with preparative purposes, to collect the amount of material to be subjected to mass-spectrometry analysis). Caveolin and flotillin positive fractions were collected and used in the 2-D gels. Molecular weights are reported on the right.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Proteomic Identification of VEGF-dependent Protein Enrichment to Membrane Caveolar-raft Microdomains in Endothelial Progenitor Cells

    doi: 10.1074/mcp.M112.024638

    Figure Lengend Snippet: 2-D gels of caveolar-raft fractions. Silver-stained 2-D gels of caveolar-raft membrane fractions from control (A) and VEGF-stimulated ECFCs (VEGF: 50 ng/ml for 12 h) (B). Proteins (40 μg) were separated by their pI on 4–7 linear IPG strips and by their molecular weight on 9–16% polyacrylamide linear gradient gels. Black circles indicate spots that presented a differential occurrence in micro-domain after VEGF stimulation. Numbers indicate spot identified by mass spectrometry. The insets of each panel show the Western blotting with anticaveolin, antiflotillin and anti-integrinβ1 antibodies of fractions obtained by density-gradient centrifugation of ECFCs lysates (the caveolin-rich fractions Western blotting in each panel is representative of 20 different experiments performed with preparative purposes, to collect the amount of material to be subjected to mass-spectrometry analysis). Caveolin and flotillin positive fractions were collected and used in the 2-D gels. Molecular weights are reported on the right.

    Article Snippet: UCB was diluted 1:1 with Hank's balanced salt solution (HBSS, EuroClone) and was overlaid on an appropriate volume of density gradient separation medium (Lympholyte®, CEDARLANE).

    Techniques: Staining, Molecular Weight, Mass Spectrometry, Western Blot, Gradient Centrifugation